Generator

Part:BBa_K1472611

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-28)

IPTG inducible expression of ∆9, ∆12, and ∆15 desaturases

It is a device that contains a promoter (BBa_R0010) and an RBS (BBa_B0034) for each of the three desaturase genes. When free fatty acid, for example, stearic acid (18:0) enters a cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linoleic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase(Figure 1). This device is designated to express in the presence of IPTG.

CityU HK newBB depmt91215b.jpg

Figure 1. Conversion of stearic acid into alpha-linolenic acid by 3 desaturases.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal BamHI site found at 673
    Illegal BamHI site found at 1638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 497
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 1723
    Illegal AgeI site found at 1861
    Illegal AgeI site found at 2694
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None